Gomal College of Veterinary Sciences, Gomal University, D. I. Khan,
¹Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan.  

Abstract

   The present study was designed to determine the quarter-wise comparative prevalence of mastitis in buffaloes and crossbred cows. Milk samples collected from 50 dairy buffaloes and 50 crossbred cows were tested for subclinical mastitis by Surf Field Mastitis Test. In addition, all milk samples were processed for isolation and identification of pathogens. In buffaloes, overall prevalence of subclinical mastitis was 27%, clinical mastitis 4% and blind quarters 10%. In crossbred cows, subclinical mastitis was observed in 36%, clinical mastitis in 5.5% and blind quarters in 8% quarters. Prevalence was higher (32%) in hindquarters of crossbred cows than those of buffaloes (29%). Among the isolates, Staphylococcus aureus showed the highest (45%) frequency, followed by Streptococcus agalactiae (23%), E. coli (18%) and Bacillus spp. (14%) in buffaloes. In case of crossbred cows, Staphylococcus aureus , Streptococcus agalactiae , E. coli  and Bacillus spp.  were isolated from 48, 30, 13 and 8% milk samples respectively.