RESTRICTION ENDONUCLEASE ANALYSIS OF PASTEURELLA MULTOCIDA FIELD ISOLATES BY Hha-I

R. MUNIR, U. FAROOQ, N. BADAR, A. KHANUM¹ AND A. WADOOD¹

Animal Health Program, Animal Sciences Institute, National Agricultural Research Centre, Park Road, Islamabad, 45500 and ¹University of Arid Agriculture, Rawalpindi, Pakistan

Abstract

    In this study the restriction endonuclease analysis (REA) technique was used for the comparison of field isolates and vaccinal strain of Pasteurella multocida using Hha-I enzyme.  On the basis of DNA finger prints, extent of homology among the field isolates and the vaccinal strain was observed. The study has provided the clear evidence that eight isolates of P. multocida used were serologically different but genotypically all were same with the restriction enzyme used.

Key words: Pasteurella multocida, haemorrhagic septicemia, DNA fingerprinting.