R. MUNIR, D. SHAHWAR¹, U. FAROOQ, I. NAWAZ, I. SHAHZAD AND A. KHANUM¹

Animal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Park Road, Islamabad, ¹University of Arid Agriculture, Muree Road, Rawalpindi, Pakistan

    Outer membrane proteins (OMPs) play a significant role in the pathogenesis of Pasteurellosis. OMPs of Pasteurella multocida (P. multocida) B:2 strain were characterized by using sodium dodecyl sulphate polyacryl amide gel electrophoresis (SDS-PAGE) technique. Molecular weights of OMPs were determined by plotting graph between R_f value and log of molecular weight. A total of 6 polypeptides ranging from 15 KDa to 91 kDa were observed which included two intense bands of 39 and 32 kDa, and four less intense bands of 91, 72, 44 and 15 kDa. OMPs, therefore, can be used to identify strain specific markers that would form a useful basis to differentiate them from field isolates of P. multocida of same serotype i.e., B:2. However, this was a preliminary type of work and further research using various field isolates as well as vaccinal strains are needed.  

Key words: Pasteurella multocida, SDS-PAGE, outer membrane protein profiling.