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Changes of Antioxidant Function and the mRNA Expression Levels of Xanthine Oxidase in Primary Chick Kidney Cell Culture Caused by Nephropathogenic Infectious Bronchitis Virus Infection
 
Weilian Liu§, Ping Liu§, Tiancheng Wang, Huayuan Lin, Qiqi Huang, Guangfu Deng, Xiaona Gao, Guohui Liu, Xiaoquan Guo*, Caiying Zhang, Huabin Cao and Guoliang Hu*
 
Clinical Veterinary Laboratory, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, People’s Republic of China
*Corresponding author: Xiaoquan Guo, xqguo20720@aliyun.com; Guoliang Hu, hgljx3818@163.com
 

Abstract   

To investigate the variations of morphological, antioxidant function and xanthine oxidase (XOD) mRNA transcription of chick kidney (CK) cells underlying the nephropathogenic infectious bronchitis virus (NIBV) infection. Following NIBV infection there was a time-dependent increase in lactate dehydrogenase (LDH) both in cells and medium. Meanwhile, NIBV infection of CK cells resulted in dysregulation of antioxidant function of CK cells, as superoxide dismutase (SOD) activity was decreased and malonaldehyde (MDA) concentration was increased in cells while there was elevation of SOD activity in medium. Furthermore, the xanthine oxidase (XOD) activity and the uric acid (UA) concentration of infected group were significantly increased both in cell and medium, when cell XOD mRNA transcription showed hight in infected group than in the control group. Taken together, our results indicated the metabolic disorder of XOD was an important pathological mechanism of NIBV infection; the results partially elucidate the potential mechanisms of hyperuricemia induced by NIBV.

Key words: Antioxidant, avian gout, Chick kidney cells, Nephropathogenic infectious Bronchitis virus, Xanthine oxidase

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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