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Effect of Sodium Butyrate in Combating the Negative Effects of Sub-Acute Ruminal Acidosis Induced Lipopolysaccharides in the Uteri of Lactating Goats
 
Muhammad Shahid Bilal1, Tianle Xu1, Zainul Aabdin1, Hongyu Dai1, Juma Ahmad Abaker1, Meena Memon1, Usman Waheed2, Alam Zeb Khan3 and Xiangzhen Shen1*
 
1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China; 2Department of Pathobiology, University of Veterinary and Animal Sciences, Sub-campus Jhang, Pakistan; 3Institute of Nutritional and Metabolic Disorders of Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing 210095, P.R. China
*Corresponding author: xzshen@njau.edu.cn
 

Abstract   

Sub-acute ruminal acidosis (SARA) is known to enhance the systemic inflammatory response that most likely occurs due to the translocation of lipopolysaccharide (LPS) from the digestive tract into the blood stream. The objective of the present study was to explore whether sodium butyrate could minimize the negative effects of high-grain diet-induced inflammation in the goat uterus and to explore the differences between high-concentrate diet alone and high-concentrate diet supplemented with sodium butyrate. Eighteen mid-lactating goats surgically installed with a rumen fistula were divided into two groups: a group receiving a high-concentrate diet (HC) (60% concentrate + 40% forage) as a control and a group receiving a high-concentrate diet with sodium butyrate (HC +Na B) (60% concentrate+40% forage+sodium butyrate) as the treatment group. The LPS concentrations in the rumen fluid were 234653 EU/ml and 431564 EU/ml in the HC +Na B and HC groups, respectively, whereas in peripheral blood, LPS concentrations were 0.013 EU/ml and 0.039 EU/ml in the HC +Na B and HC groups, respectively, indicating a significant decrease in LPS in the HC +Na B group. The concentrations of the pro-inflammatory cytokines IL-1β and IL-6 were significantly lower, with concomitant down-regulation of the expression of the inflammatory genes TLR4, MyD-88, NF-κB, TRAF-6, IL-6 and IL-1β in the uteri of HC +Na B group. Therefore, supplementing sodium butyrate with a high-concentrate diet reduced the degree of uterine inflammation and played a vital role in combating the inflammatory effects of LPS migration from the digestive tract.

Key words: High-concentrate diet with sodium butyrate, Immune gene expression, Lipopolysaccharide, Sub-acute ruminal acidosis, Uterus

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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