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Detection of Plasmid-Mediated Quinolone Resistance Genes in Swine Clinical Isolates of Escherichia coli
Wenxin Lyu1,2§, Yuefei Yang1,2§, Junzhao Li1,2 and Yanhong Wang*1,2,3
1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; 2Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China; 3Jiangsu Key Laboratory of Zoonosis, Yangzhou 225009, China; *Corresponding author:


The drug resistance was investigated in swine clinical isolates of Escherichia coli in East China, and the prevalence of plasmid-mediated quinolone resistance (PMQR) genes was detected by PCR and sequencing. Among 53 clinical E. coli isolates, 47 (88.7%) isolates exhibited resistance to one or more antimicrobial agents, with the greatest resistance in norfloxacin (69.8%) and ciprofloxacin (67.9%) and the lowest resistance in amikacin (17.0%). In total, aac(6’)-Ib, qnr and qepA were detected in 10 (18.9%), 10 (18.9%) and 1 (1.9%) of 53 E. coli isolates. The 10 qnr-positive isolates include 5 for qnrB and 5 for qnrS, whereas no qnrA and qnrD were detected. Noteworthy, 90% (9/10) aac(6’)-Ib-positve isolates were the cr variant allele. The fact that, none resistance gene was detected in some isolates with severe drug resistance, suggests that there may be other potential genes or mechanisms mediating it.

Key words: Escherichia coli, PMQR, Resistance


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)