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Antibacterial Effect of Different Concentrations of Silver Nanoparticles
A. Ebru Borum1* and Ertan Güneş2
1Department of Microbiology, Faculty of Veterinary Medicine, Balıkesir University, Balıkesir, Turkey
2Vocational School of Technical Sciences, Uludag University, Bursa, Turkey
*Corresponding author:


Silver has been in use since time immemorial in the form of metallic silver, silver nitrate, silver sulfadiazine for the treatment of burns, wounds and several bacterial infections. Silver has long been known to show a strong antimicrobial effect to microorganisms. The antimicrobial effect of 30 and 100 ppm silver nanoparticles were investigated against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Enterococcus faecalis, Bacillus cereus, Bacillus subtilis, Paenibacillus larvae, Candida albicans, Aspergillus niger. The microorganisms were diluted with sterile distilled water and prepared dilutions of 106 of test microorganisms. Dilutions of microorganisms cultured to blood agar base and incubated at 37°C for 24 hours. One ml. dilution of 106 of all of microorganisms was centrifuged at 3500 rpm for 20 minutes. 30 and 100 ppm silver solutions were added. Samples were inoculated Blood Agar at 2, 5, 10, 30, 60 minutes and 24 hour. We detected antibacterial activity of silver nanoparticles against various microorganisms in 0, 2, 5, 10, 30, 60 minute and 24 hours. As results, yeast, fungi and bacteriae were inhibited at 30 and 100 ppm. But, P.larvae was not inhibited, B. subtilis could not be inhibited at 30 ppm. The antibacterial activity of 100 ppm of nanoparticles was stronger than the antibacterial activity of 30 ppm of nanoparticles. Nanosilver is very effective to important pathogens.

To Cite This Article: Borum AE and Güneş E, 2018. Antibacterial effect of different concentrations of silver nanoparticles. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)