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Depletion of Residual Amoxicillin and Its Major Metabolites in Muscle, Liver and Kidney of Chicken
Yangyang Zhang123, Xing Xie4, Maoda Pang4, Min Zhao123, Kaizhou Xie123*, Bo Wang123, Xia Zhao123, Yajuan Wang123, Ran Wang4, Haiqing Wu123, Genxi Zhang123, Tao Zhang123, Guojun Dai123 and Jinyu Wang123
1College of Animal Science and Teczhnology, Yangzhou University, Yangzhou 225009, China;
2Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; 3Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University
4Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
*Corresponding author:


The depletion of residual amoxicillin (AMO) and its metabolites, amoxicillin acid (AMA) and 2,5-diketopiperazine (DIKETO), in Jinghai chickens was studied. Chicken tissue samples (muscle, liver and kidney) were deproteinized with acetonitrile and water and extracted with saturated dichloromethane, and the supernatants were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). After the drug was withdrawn, the depletion times of AMA and DIKETO were longer in the liver and kidneys than in muscle. In the chicken tissue, the AMA residue levels were higher than the AMO and DIKETO residue levels, and the concentrations of AMA were highest in the kidney and liver. Because AMA is an allergen, we recommend monitoring AMA levels even though maximum residue limits (MRLs) for the metabolites of AMO have not been specified. In addition, the calculated withdrawal times for AMO at doses of 30 and 60 mg/kg chicken body weight were 4.01 and 4.33 days in muscle, 5.17 and 5.78 days in the liver, and 3.92 and 5.19 days in the kidney, respectively. To guarantee food safety, AMO withdrawal times of 6 days are required for doses of 30 or 60 mg/kg chicken body weight.

To Cite This Article: Zhang Y, Xie X, Pang M, Zhao M, Xie K, Wang B, Zhao X, Wang Y, Wang R, Wu H, Zhang G, Zhang T, Dai G and Wang J, 2018. Depletion of residual amoxicillin and its major metabolites in muscle, liver and kidney of chicken. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)