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Sequestration and Histopathological Changes of the Kidneys, Lungs and Brain of Mice Infected with Plasmodium berghei that Exposed to Repeated Artemisinin
Lilik Maslachah1*, Thomas V Widiyatno2 and Lita Rakhma Yustinasari3
1Department of Veterinary Basic Medicine; 2Department of Veterinary Pathology; 3Department of Veterinary Anatomy, Veterinary Medicine Faculty of Universitas Airlangga, Surabaya, Indonesia
*Corresponding author:


The purpose of this study was to determine the pathogenesis of malarial infection in rodent as in vivo model in humans due to repeated exposure of artemisinin through organ histopathological picture. Healthy adult Albino swiss mice with average weight of 20-30 g were used for the study. Fifteen mice were divided into three groups: mice were infected with Plasmodium berghei which has been ever treated with artemisinin up to 4 times than treated by artemisinin (T4), infected mice with Plasmodium berghei which untreated by artemisinin as a control (C), infected mice with Plasmodium berghei which has been ever treated by artemisinin 4 times but untreated as a treatment control (TC). T4 group was oral administered with artemisinin which was given with "4-day-test" (4-DT) with ED99 dose (200 mg/kg weight of mice) for 3 days which begins 48 hours after infection but C and TC group were given aquadest. The histopathology of the lung, kidney, and cerebrum tissues was studied by routine histology method with Haematoxylin-Eosin staining. Histological examination edema, haemosiderosis, thickened alveolar septa and inflammatory cell infiltration in the lung. Cast formation Glumerulonephritis, tubular necrosis, and congestion occurred in the cortex area of the kidney. The brain showed cerebral microvessels congested, haemorrhages and necrosis. Conclusions repeated artemisinin exposure with repeated passages in mice cause increasing of sequestration on the brain and lungs and increasing the histopathological changes of the lung, kidney, and cerebrum.

To Cite This Article: Maslachah L, Widiyatno TV and Yustinasari LR, 2019. Sequestration and histopathological changes of the kidneys, lungs and brain of mice infected with Plasmodium berghei that exposed to repeated artemisinin. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)