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Matrine Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by Influencing the Activation of Nsp9
 
Panpan Sun1, Na Sun1, Zhirui Wang2, Jianhua Guo3, Yongming He4* and Hongquan Li1*
 
1College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, P.R. China; 2Center for Transplantation Sciences, Massachusetts General Hospital and Harvard Medical School, Boston MA, USA; 3Department of Veterinary Pathobiology, Schubot Exotic Bird Health Center, Texas A&M University, College Station, TX 77843, USA; 4School of Life Science and Engineering, Foshan university, Foshan, Guangdong 528231, P.R. China
*Corresponding author: livets@163.com; ymhe@fosu.edu.cn
 

Abstract   

Matrine is the major active compound found in the root of Chinese herbal medicine Sophora flavescens and has a wide range of pharmacological efficacies. Our previous studies showed that Matrine could inhibit the replication of porcine reproductive and respiratory syndrome virus (PRRSV) directly. However, the underlying inhibition mechanism remains unknown. The main aim of this study was to explore the inhibitory mechanism of Matrine in PRRSV replication in vitro. The mRNA expression of PRRSV N gene was determined using RT-qPCR. The expression of pERK1/2 protein was evaluated using Western blot and the effect of Matrine on PRRSV Nsp9 activity was studied using dual luciferase reporter gene assay. The results showed that Matrine suppress the mRNA transcription of PRRSV N gene in Marc-145 cells at 3 h and 24 h post PRRSV infection and had no inhibitory effect on the ERK1/2 signal pathway activated by PRRSV. The results of dual luciferase reporter gene assay showed that Matrine suppressed the interaction of Nsp9 with promoter p107. Taken into account the results above, our study demonstrated that Matrine inhibit the activity of Nsp9 to suppress the virus replication.

To Cite This Article: Sun P, Sun N, Wang Z, Guo J and Li H, 2018. Matrine Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by influencing the activation of Nsp9. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2018.079

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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