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Association of Bovine Tumor Necrosis Factor Alpha Gene Polymorphism with Mastitis in Nili Ravi Buffaloes
Sehrish Firyal1*, Samia Tanveer1, Ali Raza Awan1, Muhammad Tayyab1, Muhammad Wasim1, Muhammad Nawaz2, Huma Sattar1, Sadia Nawaz1, Shagufta Saeedand Muhammad Muddassir Ali1
1Institute of Biochemistry and Biotechnology; 2Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan; *Corresponding author:


The current research was designed to find out polymorphic changes in complete gene sequence of tumor necrosis factor alpha (TNF-α), which is involved in inducing mastitis in Nili Ravi buffaloes. Total 30 blood samples were collected having clinical (n=10), subclinical (n=10) mastitis and normal (n=10) animals. DNA was extracted, TNF-α gene was amplified and sequenced. Homology analysis of TNF-α gene sequences using Finch TV tool revealed total of eleven SNP’s in subclinical mastitic buffaloes and twelve in clinical mastitic buffaloes, including insertion, heterozygosity, transitional and some silent mutations; in intronic region. In subclinical mastitis samples, insertion of “G” at the position 279, transition mutation of (A/G) at position 1022, Heterozygosity of (A/G) at 1023 cite, transition mutation of (A/G) at position 1129, deletion of “C” at position 1741 and transition of (A/G) were found at cite 2091 of this gene, respectively. In the clinical mastitis samples, there was an insertion of “G” at the position 279, transition mutation of (A/G) at position 1022 and Heterozygosity of (A/G) at 1023 cite were found. All these SNPs were not found in sequences of normal animals. The Current genome association study showed the potential correlation between these significant polymorphisms and incidence of clinical and subclinical mastitis in Nili Ravi buffaloes.

To Cite This Article: Firyal S, Tanveer S, Awan AR, Tayyab M, Wasim M, Nawaz M, Sattar H, Nawaz S, Saeed S and Ali MM, 2019. Association of bovine tumor necrosis factor alpha gene polymorphism with mastitis in Nili Ravi buffaloes. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)