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Protective Effects of Salidroside and Dexamethasone against E.coli-Induced Inflammatory Response on Endometrial Epithelium Cells in Yaks
Hailong Dong1, Muhammad Ijaz2, Khalid Mehmood3, Muhammad Muddassir Ali4, Fayi Tian1, Jiakui Li1, Shakeel Ahmed5, Aftab Shaukat6, Zhenyu Chang1* and Qingxia Wu1*
1Animal Science College, Tibet Agriculture & Animal Husbandry University, Linzhi 860000, Tibet, People's Republic of China; 2Department of Clinical Medicine & Surgery, University Veterinary and Animal Sciences Lahore-54000, Punjab, Pakistan; 3University College of Veterinary & Animal Sciences, the Islamia University of Bahawalpur, 63100, Pakistan; 4Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, 54600, Pakistan; 5Lab of Drug Discovery and Molecular Engineering, Department of Medicinal Plants, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan  430070, P.R. China; 6Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P.R. China.
*Corresponding author:;


Endometritis is an inflammation of the uterus, which can reduce the reproductive performance in yaks. Salidroside is a natural monomer of Rhodiolarosea, it has anti-inflammatory, antioxidative and immune regulation properties. We conduct this study to investigate the effect of salidroside and dexamethasone on E. coli-induced inflammatory response on endometrial epithelium cells in yaks. For this purpose, the endometrial epithelial cells were cultured and dived into four groups: control group, E.coligroup, salidroside group and dexamethasone group. The antioxidant response, cytokine assays and immunofluorescence staining analysis were performed. The results showed that the intracellular activities of SOD were decreased, while MDA contents were increased significantly in E.coli-induced inflammation group. Whereas, the MDA contents decreased and SOD increased in salidroside and dexamethasone supplement groups. Moreover, E. coli results in significantly increase in cytokine production. The TNF-α, IL-1α, IL-6 and IL-10 levels in the cells treated with salidroside and dexamethasone were decreased significantly compared to those of the E.coli group. E.coli-induced inflammatory cells showed an increase in the level of TNF-α protein compared to control group. The salidroside administration reduced the TNF-α expression significantly just like dexamethasone. In conclusion, salidroside and dexamethasone play important role in regulating E.coli-induced inflammatory response in endometrial epithelium cells. Furthermore, salidroside treatment is encouraged for the control of endometritis, which may target to reduce the incidence of endometritis.

To Cite This Article: Dong H, Ijaz M, Mehmood K, Ali MM, Tian F, Li J, Ahmed S, Shaukat A, Chang Z and Wu Q, 2018. Protective effects of salidroside and dexamethasone against E.coli-induced inflammatory response on endometrial epithelium cells in yaks. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)