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Incidence of ESBL-Producing-Escherichia coli in Poultry Farm Environment and Retail Poultry Meat
 
Sadeeq ur Rahman1*, Shakoor Ahmad2 and Imad Khan1
 
1College of Veterinary Sciences and Animal Husbandry, Section Microbiology, Abdul Wali Khan University, Mardan-Khyber Pakhtunkhwa Pakistan; 2Department of Animal Health, The University of Agriculture, Peshawar – Khyber Pakhtunkhwa Pakistan; *Corresponding author: sadeeq@awkum.edu.pk
 

Abstract   

Antimicrobials are excessively used in Pakistan during broiler poultry production, however, status of antimicrobial resistance (AMR) is not known. We report on the  frequency of extended spectrum β-lactamase (ESBL) –producing-Escherichia coli recovered from samples obtained from poultry farm environment (n=100) and chicken meat samples (n=100) collected at sales point of live bird market in District Peshawar, Pakistan. Phenotypically confirmed ESBL producers were further investigated for the presence of ESBL- and carbapenemase-encoding genes and types of integrons. Our results showed that 38.8% (14/36) and 47.6% (20/42) of E. coli recovered from farm environment and chicken meat, respectively, were found to be ESBL producers. PCR based results showed that 30% (6/20), 10% (2/20) and 20% (4/20) of these E. coli from chicken-meat were harboring blaCTXM, blaCTXM-1 and blaSHV2, respectively, while 20% were carrying blaOXA-48 and blaNDM -1.  On the other hands, 28.5% (4/14), 28.5% (4/14) and 14.2% (2/14) of E. coli isolated from chicken farm-environment were harboring blaCTXM, blaCTXM-1 and blaSHV2, respectively, while, 28.5% (4/14) and 14.2% (2/14) were carrying blaOXA-48 and blaNDM -1. Integron 2 was PCR amplified from 17 isolates and integron 1 from 16 isolates, while, integron 3 was absent from all. Finally, insertion sequence ISCR1 was PCR-amplified from 15 isolates (41.6%), which was found associated with blaCTXM among 6 (16.6%) isolates suggesting its role in mobilization. Overall, our results suggest a high rate of occurrence of ESBL- and carbapenemase-carrying E. coli in poultry farm environment and chicken meat.

To Cite This Article: Rahman SU, Ahmad S and Khan I, 2018. Incidence of Esbl-producing-escherichia coli in poultry farm environment and retail poultry meat. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2018.091 

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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