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Molecular Identification of Brucella abortus Collected from Whole Blood Samples of Seronegative Dairy Cattle with Reproductive Disorders in Central Java, Indonesia
 
Hastari Wuryastuty1*, Raden Wasito2 and Sugiyono2
 
1Department of Internal Medicine; 2Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia; *Corresponding author: hastari@ugm.ac.id
 

Abstract   

Brucellosis is a zoonotic disease that led to economic losses to cattle industries worldwide including Indonesia. A rapid, precise and accurate diagnosis technique is required for early detection and for the control of brucellosis in animals and humans. The objective of this study was to molecularly identify and characterize B. abortus in the blood samples obtained from seronegative dairy cattle that have had reproductive disorders in Central Java, Indonesia, using PCR, phylogenetic and nucleotide sequence analyses of the IS711 regions. Results showed that in seronegatively dairy cattle with reproductive disorders, B. abortus was successfully detected from the whole blood and serum samples without separation of the buffy-coat and without isolation of the organism by conventional PCR. The result also showed high homology level (up to 100%) and close phylogenetic relationships between nucleotide sequences of the IS711 gene fragment of B. abortus local isolates compare to the isolates that have been accessed in GenBank (CP 009099, CP 023242, CP 023242, CP 023308 and LT 671513). We concluded that PCR technique is useful for routine diagnosis of brucellosis in seronegative dairy cattle with reproductive disorders.

To Cite This Article: Wuryastuty H, Wasito R and Sugiyono, 2019. Molecular identification of Brucella abortus collected from whole blood samples of seronegative dairy cattle with reproductive disorders in central java, Indonesia. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2019.010

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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