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Tracing of Salmonella Contaminations Throughout an Integrated Broiler Production Chain in Dakahlia Governorate, Egypt
 
Mona Elsayed1, Fatma El-Gohary1, Amira Zakaria2 and Mayada Gwida1*
 
1Department of Hygiene and Zoonosis, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
2Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
*Corresponding author: mayada_gwida@mans.edu.eg; mayada.gwida@gmail.com
 

Abstract   

The data available regarding the production chain particularly in poultry flocks in Egypt are yet to be fully elucidated. The study aimed at providing an insight into the cross-contamination with Salmonella spp. at three different stages of an integrated broiler production chain. From 440 collected samples, 152 (34.5%) were positive for Salmonella spp. corresponded to samples collected from broiler farms (n=90, 40.9%), slaughter houses contact surfaces (n=12, 24%) and chicken carcass (n=50, 29.4%) in an integrated broiler supply chain. Isolation and identification of salmonella spp. were followed the standard procedures. Genomic bacterial DNA was extracted and confirmed using specific oligonucleotide primers sequences. Salmonella strains were classified into 11 serotypes. Nine different serotypes were found to contaminate thebroiler houses and flocks with the most prevalent serotypes S. Enteritidis (38.8%), S. Kentucky (23.3%) and S. Typhimurium (11.11%). The overall frequency of Salmonella contamination in the live broiler flocks was 40.9% with prevalence of 37.9, 60, 40, 53.3, 60 and 20% from cloacae swabs, litter, feed, water, farms walls and in the hand swabs from workers, respectively. The PCR products of four isolates, (two isolates per S. Typhimurium and S. Enteritidis) were identified by DNA sequencing to determine the potential genetic relationships in these isolates. The findings of the present study suggested that different Salmonella serotypes can persist along the production chain and that contamination could be linked to the contamination of the final product with a potential health hazard to consumers.

To Cite This Article: Elsayed M, El-Gohary F, Zakaria A and Gwida M, 2019. Tracing of salmonella contaminations throughout an integrated broiler production chain in Dakahlia Governorate, Egypt. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2019.038

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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