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Investigation of Different Serotypes of FMDV in Vaccinated Buffaloes (Bubalus bubalis) in Southern Areas of Punjab Province, Pakistan
 
Riaz Hussain1*, Fazal Mahmood2, Bilal Aslam3, Abu Baker Siddique3, Azhar Rafique4, Syed Abdul Khaliq5, Iahtasham Khan6, Sadaf Imran7, Mansoor Mubeen8, Jahanzaib9 and Azam Ali Nasir10
 
1University College of Veterinary and Animal Sciences, the Islamia University of Bahawalpur; 2Department of Pathology, Faculty of Veterinary Sciences, University of Agriculture, Faisalabad; 3Department of Microbiology, Faculty of Science and Technology, Government College University, Faisalabad; 4Department of Zoology, Government College University, Faisalabad; 5Animal Disease Diagnostic Reporting and Surveillance, 16 Cooper Road, Lahore; 6Section of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore Sub-Campus, Jhang; 7Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences Lahore; 8Livestock Production Research Institute, Bahadurnagar, Okara; 9District disease diagnostic laboratory Lodhran, Livestock and Dairy Department Punjab; 10Veterinary Research Institute Lahore, Pakistan; *Corresponding author: driazhussain@yahoo.com
 

Abstract   

Foot and mouth disease (FMD) is highly endemic in Pakistan, which induces heavy economic loss to livestock holders in term of decrease milk production, high morbidity and mortality in large and small ruminants. In present study, we observed the clinico-pathological lesions during an outbreak of FMD in vaccinated buffaloes. Overall 31.56% morbidity (119/377), 4.77% mortality (18/377) and 15.12% case fatality (18/119) was recorded during the current outbreak. The morbid animals were lethargic, depressed and exhibited marked lameness, profuse salivation, myositis of tongue, vesicular fluid and epithelial sloughing. Vesicular fluid and epithelial sloughing collected from clinically sick animals were positive for FMDV predominantly O strain and Asia I through ELISA technique. Grossly, mandibular lymph nodes were swollen and hemorrhagic in infected animals. The mucosa of abomasum was severely congested, ulcerated and showed hyperemic edges with centrally yellow necrotic areas. Severe purulent inflammation of jejunum and petechial hemorrhages over base of heart were also observed. Histologically degenerative changes in keratinocytes in stratum spinosum, marked acanthosis and intracellular accumulation of eosinophilic, a cellular, transudate forming characteristic vesicles and bullae were observed in epidermis. The mandibular lymph nodes exhibited prominent capillaries engorged with erythrocytes, necrosis and dendritic cells with engulfed necrotic cells. Hemosiderin accumulation was also observed. Fusion and stunting of intestinal villi along with infiltration of inflammatory cells were characteristic lesions in infected animals. From the findings of our study it can be suggested that clinical signs, necropsy lesions and histopathological changes are valuable and useful tools for the diagnosis of foot and mouth disease in Bubalus bubalis. Moreover, strict surveillance, use of good quality vaccines, regular monitoring and geographical distribution of various serotypes of FMDV are valuable tool for establishment of effective control procedures.

To Cite This Article: Hussain R, Mahmood F, Aslam B, Siddique AB, Rafique A, Khaliq SA, Khan I, Imran S, Mubeen M, Jahanzaib and Nasir AA, 2019. Investigation of different serotypes of FMDV in vaccinated Buffaloes (Bubalus bubalis) in Southern Areas of Punjab Province, Pakistan. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2019.062

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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