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Effect of Newly Characterized Probiotic Lactobacilli on Weight Gain, Immunomodulation and Gut Microbiota of Campylobacter jejuni Challenged Broiler Chicken
 
Madiha Khan1, Aftab A Anjum1*, Muhammad Nawaz1, Ali Raza Awan2 and Muhammad Asad Ali1
 
1Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan
2Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
*Corresponding author: aftab.anjum@uvas.edu.pk
 

Abstract   

The aim of the present study was to determine the competitive exclusion of Campylobacter jejuni in poultry gut by three potential probiotic lactobacilli strains. A total of 135 birds of one day age were randomly divided into nine groups. The groups of prevention model received respective strains from day 1-35 and treatment model received lactobacilli from day 15-35 (after challenging with C. jejuni). These groups were given lactobacilli (~108 CFUs) while challenged with C. jejuni on day 14 by oral gavage (106 CFUs/bird). There were three control groups including A, B and I. Negative control (A) did not receive any treatment, C. jejuni was given to group B and group I was given Enrofloxacin formulation. Cloacal swabs were collected from birds of each group before and after challenge while the ceca were collected from birds after slaughtering (on day 35) for enumeration of aerobic bacteria, coliform, C. jejuni, lactobacilli and Bifidobacterium on selective agar plates. Effect of lactobacilli on weight gain and New Castle Disease vaccine (NDV) titer were also evaluated. Probiotic strain Lactobacillus gallinarum PL 53 considerably decreased log10 values of aerobic plate count (3.19±0.66), coliform count (2.83±0.22) and C. jejuni (3.98±0.77) in poultry. The probiotics also enhanced Lactobacillus and Bifidobacterium spp counts (~2-3 log increase). Treatment group C had maximum weight gain (1994±188.32g) and geometric mean titer (274.4) on day 28. The results of in-vivo experiments concluded that probiotic administration may be effective for targeted mitigation of C. jejuni in broiler birds.

To Cite This Article: Khan M, Anjum AA, Nawaz M, Awan AR and Ali MA, 2019. Effect of newly characterized probiotic lactobacilli on weight gain, immunomodulation and gut microbiota of Campylobacter jejuni challenged broiler chicken. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2019.051

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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