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Phenotypic and Genotypic Assortment of Polyhydroxyalkanoates Producing Bacteria from Rumen Flora of Domesticated Animals in Pakistan

A Rafique1*, MS Mahmood2, A Ashraf1, S Nasir1, M Kamran2, N Jamil3, F Jabeen1, T Sultana1, S Sultana1, JA Khan4, MLuqman2 and A Shamim4

1Department of Zoology, GC University Faisalabad; 2Institute of Microbiology, University of Agriculture, Faisalabad
3School of Biological Science, Punjab University, Lahore; 4Institute of Physiology, Pharmacology and Pharmacy, University of Agriculture, Faisalabad; 4Department of Pathobiology, Faculty of Veterinary & Animal Sciences, University of the Poonch, Rawalakot, Azad Kashmir, Pakistan; *Corresponding author:


In plastic industry, a lot of attention has been gained by Polyhydroxyalkanoates (PHAs) owing to their environment friendly nature and 100% biodegradable, so they are called as “green plastics”. Bacterial strains able to produce PHAs under veterinary field are reported and rumen microflora was exploited to check its potential for the PHAs production. From the rumen of domestic cattle samples were collected followed by isolation of PHA producing bacteria. Biochemical and molecular characterization of isolates was done through API 20E kit and polymerase chain reaction (PCR), respectively. In order to confirm the genetic diversity, sequencing of 16S rRNA gene, ribotyping and phylogenetic analysis was performed. A deeper insight of PHAs biopolymer by Fourier transform infrared spectroscopy (FTIR) revealed the functional groups. Pseudomonas aeruginosa (P. aeruginosa) was isolated and biochemically confirmed through API 20E. PCR further confirmed P. aeruginosa through amplification of 16S rRNA gene. Sequenced data of 16S rRNA (accession# MN625895) analyzed through ribotyping and phylogenetic linkages proved all the isolate belongs to P. aeruginosa. PHAs were purified from enriched nutrient broth with rumen mixture by using sodium hypochlorite confirmed through FTIR spectroscopy. Complex accumulation of bacterial biomass showed -C=O, C-H and -CH3 functional groups. Bacterial biomass (PHAs) in enriched carbon medium can be used as bioplastics for domestic purpose with less harm. It will be a better alternative to chemically synthesized plastic to reduce environmental hazards.

To Cite This Article: Rafique A, Mahmood MS, Ashraf A, Nasir S, Kamran M, Jamil N, Jabeen F, Sultana T, Sultana S,   Khan JA, Luqman M and Shamim A, 2019. Phenotypic and genotypic assortment of Polyhydroxyalkanoates producing bacteria from rumen flora of domesticated animals in Pakistan. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)