PAKISTAN
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Characterization and Genome Analysis of a Novel Escherichia coli Bacteriophage vB_EcoS_W011D
 

Xinwu Wang, Hengyu Xi, Jizuo Su1, Mengjun Cheng2, Gang Wang1, Dali He1, Ruopeng Cai1, Zijing Wang1, Yuan Guan1 Changjiang Sun1, Xin Feng1, Liancheng Lei1, Sadeeq ur Rahman3, Jianbao Dong4*, Wenyu Han1,5* and Jingmin Gu1,2*

 
1Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China; 2Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, P.R. China; 3College of Veterinary Sciences & Animal Husbandry, Abdul Wali Khan University, Mardan 23200, Pakistan; 4Department of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang 261061, P.R. China; 5Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou 225009, P.R. China
*Corresponding author: jingmin0629@163.com (JMG); hanwy@jlu.edu.cn (WYH); djb922@hotmail.com (JBD)

Abstract   

The application of phages against the increased reported drug resistant Escherichia coli is one of the promising alternative therapeutic options. The total number of phages on the earth are more than 1031, but the phages that have been isolated and studied are limited. Hence, discovering of new phage and uncovering its characteristics will provide materials for extensive use of phage therapy in the future. In this study, a novel E. coli phage named vB_EcoS_W011D was isolated and the characteristics and genome were explored. The typical morphology of vB_EcoS_W011D is comprised of an icosahedral head and a constricted flexible rolled up tail, revealing that it is the genus TLS virus of Tunavirinae subfamily. One-step growth curve showing the eclipse and latent period of vB_EcoS_W011D was 5 min and 10 min, respectively, with the burst size of 115 PFU/cell. The genome of vB_EcoS_W011D is double-stranded consisting of 49,847 bp with 46.24% of G+C contents and shows 77% similarities (with 38% query coverage) to other reported phages. A total 85 putative ORFs were identified. Of which, 43 predicted ORFs had significant homology with other phage proteins of known functions. A putative Zonula occludes toxin was found in its genome. In addition, a clear difference was revealed on the phylogenetic analysis of it terminates large subunit and capsid protein. In conclusion, our study clearly indicates that vB_EcoS_W011D is a newly discovered E. coli phage that could be further investigated to elucidate phage variety and evolutionary relationship between bacteria and phages.

To Cite This Article: Wang X, Xi H, Su J, Cheng M, Wang G, He D, Cai R, Wang Z, GuanY, Sun C, Feng X, Lei L, Rahman SU, Dong J, Han W and Gu J, 2019. Characterization and genome analysis of a novel Escherichia coli bacteriophage vB_EcoS_W011D. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2019.114

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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