PAKISTAN
VETERINARY
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Developing a Novel, Efficient and Cost Effective Tissue Injury Model (in-vitro) on Equine Tendon Fibroblasts
 
Somia Shehzadi1, Maryam Javed1*, Sana Javaid Awan2, Asif Nadeem1,4 and Tahir Yaqub3
 
1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
2Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan
3Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan
4Department of biotechnology, Virtual University of Pakistan, Lahore
*Corresponding author: maryam.javed@uvas.edu.pk

Abstract   

Tendinopathy or tendon degeneration in equine is an illness caused by inflammation or injury. Being a difficult tissue to repair, tendon damage can cause physical disabilities. Successful tendon regeneration requires an appropriate in-vitro model for tissue injury. Current study was planned to compare the effect of two inflammatory mediators: prostaglandinE2 (PGE2) and hydrogen peroxide (H2O2) on equine tendon fibroblasts. Tendon fibroblast cultures were subjected to two different doses of PGE2 (100ng/ml and 200ng/ml) and H2O2 (500µM and 1mM). Compared with untreated tendon fibroblasts, all doses of PGE2 and H2O2 reduced cell viability markedly. Viability was assessed by trypan blue staining method and crystal violet staining. Lactate dehydrogenase (LDH) release was measured to assess injury and cell proliferation by MTT assay. In comparison of dose, 100ng/ml of PGE2 and 500µM of H2O2 showed optimal results, whereas, higher dose exhibited highly reduced cell viability and increased cytotoxicity, which may cause difficulty in studying tendon injury in-vitro model. These findings suggest that both PGE2 and H2O2 have equal potential to cause tendon fibroblasts injury. Therefore, H2O2 can be used as cost-effective in-vitro model for tendon degeneration studies.

To Cite This Article: Shehzadi S, Javed M, Awan SJ, Nadeem A and Yaqub T, 2020. Developing a novel, efficient and cost effective tissue injury model (in-vitro) on equine tendon fibroblasts. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2020.058

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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