PAKISTAN
VETERINARY
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Phenotypic and Genotypic Characterization of Beta-lactams Resistant Staphylococcus aureus Isolates from Bovine Mastitis and its Zoonotic Implications
 
Ammara Khan1, Sehrish Firyal1*, Iahtasham Khan2, Ali Raza Awan1, Muhammad Tayyab1, Muhammad Wasim1, Muhammad Zubair Shabbir3, Muhammad Muddassir Ali1 and Ali Zohaib4*
 
1Institute of Biochemistry and Biotechnology; 2Section of Epidemiology and Public Health, University of Veterinary and Animal Sciences, sub-campus Jhang; 3Department of Microbiology, University of Veterinary and animal sciences, Lahore, Pakistan; 4Department of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Bio-Sciences, National University of Sciences and Technology (NUST), Islamabad, Pakistan
*Corresponding author: alizohaib7@hotmail.com; sehrishfiryal@uvas.edu.pk

Abstract   

Rise of antibiotic resistant Staphylococcus aureus is a higher risk and great concern to global health. Zoonotic transfer of such strains is well documented. Present study evaluated the presence of resistant S. aureus from mastitic Nilli Ravi buffaloes and nasal carriage of milkers. Phenotypic profile of S. aureus isolates was conducted against penicillin, ampicillin, and cefoxitin. PCR analysis revealed presence of blaZ gene and mecA gene from S. aureus isolated from milk and milkers samples. Sequence and phylogenetic analysis depicts the divergence of mecA gene originated from bovine and human but for the blaZ gene, no divergence was detected. The high degree of genetic relatedness among blaZ and mecA genes in bovine and human S. aureus isolates from same farm suggests the potential transfer of antimicrobial resistance genes between buffaloes and milkers, highlighting the importance of one-health approach to promote global health.

To Cite This Article: Khan A, Firyal S, Khan I, Awan AR, Tayyab M, Wasim M, Shabbir MZ, Ali MM, Zohaib A, 2020. Phenotypic and Genotypic Characterization of Beta-lactams Resistant Staphylococcus aureus Isolates from Bovine Mastitis and its Zoonotic Implications. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2020.065

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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