PAKISTAN
VETERINARY
JOURNAL
     
 
previous page   Pak Vet J, xxxx, xx(x): xxx-xxx   next page
 
Molecular Epidemiology and Characteristics of Streptococcus agalactiae Isolated from Bovine Mastitis in Large Dairy Herds of China
 

Xueyun Yang1,2, Dengfeng Wang2, Jianjun Li2, Xiaoxiao Meng2, Yurong Wei2, Gunuer Tuerxun2, Yinghong Jin2, Jing Xue2, Tariq Ali3,4, Bo Han4* and Bin Jia1*

 
1College of Animal Science and Technology, Shihezi University, Shihezi 832003, China; 2Institute of Veterinary Medicine, Xinjiang Academy of Animal Sciences, Urumqi 830013, China; 3Veterinary Research Institute Peshawar, Khyber Pakhtunkhwa, Pakistan; 4College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
*Corresponding author: hanbo@cau.edu.cn; jiabin@shzu.edu.cn

Abstract   

Streptococcus agalactiae is one of the important causative agents of bovine mastitis. Studies on molecular epidemiology, virulence factors and antimicrobial susceptibility profiles of S. agalactiae isolates from mastitis in China are scarce. Thus, this study was carried out to investigate prevalence of S. agalactiae associated with subclinical mastitis, to determine antimicrobial susceptibility profile, and to analyze their phenotypic and genotypic profiles. Capsular serotypes and genotypes by multilocus serotyping and virulence genes (hemolysin III, C-β protein, C-α protein, surface protein rib, hyaluronate lyase, and C5a peptidase) were determined using molecular assays. Additionally, susceptibility of S. agalactiae isolates to antimicrobial agents was accessed through standard disc diffusion method. A total of 2225 milk samples were collected from 21 large dairy herds located in 10 provinces of China. Overall, 133 (14.1%) S. agalactiae isolates were recovered from 946 (42.5%) subclinical mastitis milk samples. Serotype Ia and ST103 were the most prevalent serotypes and genotypes. Five of the six virulence genes were detected in 22.6% isolates with only two virulence genes and 6.8% of isolates with three genes. There were no significant geographical differences in the distribution of virulence genes. Moreover, 63.9% of S. agalactiae exhibited resistance to levofloxacin, 3.8% isolates were resistant to cefepime, 2.3% were resistant to chloramphenicol and ampicillin. Additionally, two ampicillin/cefotaxime/levofloxacin-resistant isolates, one vancomycin-resistant isolate and one ampicillin/cefotaxime-resistant isolate were identified. This study concludes high prevalence of S. agalactiae from subclinical mastitis carrying virulence genes and mainly belonging to Serotype Ia and ST103.

To Cite This Article: Yang X, Wang D, Li J, Meng X, Wei Y, Tuerxun G, Jin Y, Xue J, Ali T, Han B and Jia B, 2020. Molecular epidemiology and characteristics of Streptococcus agalactiae isolated from bovine mastitis in large dairy herds of China. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2020.025

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



scopus
 
DOI
 
DOAJ SEAL