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Evaluation of Passive Immunity Transfer against G6P[1] Rotavirus in Holstein Calves by ELISA
 

Thaís G Rocha1*, Ketherson R Silva2, Fernanda D F Silva3, Amauri A Alfieri4, Daniela G Silva1, Hélio José Montassier2, Maria da Glória Buzinaro5, Fábio Gregori3, Luiz Francisco Zafalon6 and José Jurandir Fagliari1

 
1Research Laboratory of the Department of Veterinary Medicine and Surgery, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp). Via de Acesso Prof. Paulo Donato Castellane, s/n. Jaboticabal, SP. CEP14884-900, Brazil; 2Immunology and Virology Laboratory of the Department of Pathology, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp). Via de Acesso Prof. Paulo Donato Castellane, s/n. Jaboticabal, SP. CEP14884-900, Brazil; 3Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo (USP). Av. Prof. Dr. Orlando Marques de Paiva, 87. São Paulo, SP, CEP 05508-900. Brazil; 4Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina. Londrina - PR, Brazil. Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10.011, Londrina, PR. CEP 86057-970, Brazil; 5Department of Preventive Veterinary Medicine, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp). Via de Acesso Prof. Paulo Donato Castellane, s/n. Jaboticabal, SP. CEP14884-900, Brazil; 6Research and Development, Embrapa Southeast Livestock, Rodovia Washington Luiz Km 234 s/n, Fazenda Canchim, São Carlos, SP 13560-970, Brazil
*Corresponding author: thaisgrocha@yahoo.com.br

Abstract   

The main strategy to prevent bovine rotavirus group A (RVA) diarrhea in calves is to vaccinate late-term dams aiming to enhance passive immunity transfer of specific immunoglobulins against the virus. This study aimed to evaluate influence of parity in titers of immunoglobulin G (IgG), IgG1 and IgM in serum and colostrum of vaccinated or unvaccinated Holstein cows and in serum of its calves, associated with monitoring for RVA diarrhea in calves. Cows and its calves were allotted into groups according to parity and vaccination (primiparous/multiparous; vaccinated/ unvaccinated) and serum and colostrum samples of cows were taken as well as serum and fecal samples of its calves. Parturition influenced colostral titers of IgG and IgG1, which were higher in multiparous cows, whilst IgM titers were influenced by vaccination, being higher in colostrum of vaccinated dams. Lowest serum titers of IgG and IgG1 were found in calves born to unvaccinated primiparous dams. Eleven calves presented RVA diarrhea, and genotypes G6P[11] and G6P[5] were found in the vaccinated and unvaccinated herds, respectively. Vaccination of dams prolongs humoral immunity in calves and enhances colostrum quality and should be a primary concern in primiparous cows.

To Cite This Article: Rocha TG, Silva KR, Silva FDF, Alfieri AA, Silva DG, Montassier HJ, Buzinaro MG, Gregori F, Zafalon LF and Fagliari JJ, 2020. Evaluation of passive immunity transfer against G6P[1] rotavirus in Holstein calves by ELISA. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2020.031

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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