PAKISTAN
VETERINARY
JOURNAL
     
 
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Prevalence of Antibiotic-Resistant and Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Chicken Meat from Eastern Turkey
 
Alper Baran1*, Mehmet Cemal Adigüzel2 and Mehmet Yüksel3
 
1Department of Food Quality Control and Analysis, Erzurum Vocational School, Atatürk University, Erzurum, Turkey
2Department of Microbiology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
3Department of Food Processing, Hınıs Vocational School, Atatürk University, Erzurum, Turkey
*Corresponding author: alper.baran@atauni.edu.tr

Abstract   

This study examined the antimicrobial resistance of 105 Escherichia coli isolates from broiler meat obtained from supermarkets in Erzurum, Turkey. Antimicrobial resistance profile of the isolates was done as proposed by the Clinical & Laboratory Standards Institute. Multiplex PCR was used for the presence of extended-spectrum beta-lactamase (ESBL) and determination of the phylogenetic groups in the isolates. The results showed that resistance to penicillin was the highest (97%), while resistance to carbapenems was not observed in any isolates. A high percentage (94.29%) of multidrug-resistant isolates was observed.  A total of 43 (52.14%) E. coli isolates was determined to be positive for ESBL. ESBL-producing E. coli isolates predominantly carried genes of the CTX-M class (28/43, 65.12%), followed by the TEM (26/43, 60.47%) and SHV (1/43, 2.33%) classes. The prevalence of CTX-M class isolates belonging to the CTX-M-1, CTX-M-9, CTX-M-2, and CTX-M-8/25 groups was 41.86%, 16.28%, 9.3%, and 9.3 %, respectively. The phylogenetic groups B1 (37.21%) and A0 (23.26%) were the most frequently detected. These findings show that raw chicken meat sold in Erzurum is highly contaminated with antibiotic-resistant E. coli, including ESBL-producing E. coli, which poses a serious risk for human health.

To Cite This Article: Baran A, Adigüzel MC and Yüksel M, 2020. Prevalence of antibiotic-resistant and extended-spectrum beta-lactamase-producing Escherichia coli in chicken meat from Eastern Turkey. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2020.047

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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