Bioinformatics Analysis of JAZF1 Gene in Broilers with Ascites
Syndrome
FengPing Guo1§, Pei Liu1§, Xiaoquan Guo1,
Guyue Li1, Sufang Cheng2, Zhenxing Zou3,
Xiaolu Hou4,
Vincent Latigo1, Lin Li1, Guoliang Hu1*
and Ping Liu1*
1Jiangxi
Provincial Key Laboratory for Animal Health, Institute of Animal
Population Health, College of Animal Science and Technology, Jiangxi
Agricultural University, Nanchang, Jiangxi 330045, China;
2Jiangxi
Biological Vocational College Nanchang jiangxi 330200 China; 3Hunan
Environmental Biological Vocational and Technical College, Hengyang
421005, China; 4Guangxi Agricultural Vocational College,
Nanning Guangxi 530007, China §The
authors contributed equally to this work.
*Corresponding author:
pingliujx@163.com;
hgljx@163.com
Abstract
Pulmonary vascular remodeling (PVR) is the main characteristic lesion of ascites
syndrome (AS) in broilers JAZF1 plays an important role in PVR, but there is no
study on its protein function and structure. In this study, the physical and
chemical properties, hydrophilicity / hydrophobicity and transmembrane domain,
phosphorylation site and glycosylation site, subcellular localization and signal
peptide, secondary and tertiary structure, antigen peptide and conserved domain
and phylogenetic relationship of JAZF1 protein were predicted online by
bioinformatics tools. The results showed that the number of amino acids of JAZF1
was 243aa, the theoretical isoelectric point was 8.63, the instability index was
58.1, and the average coefficient of hydrophilicity was -0.674. It was found to
be a hydrophilic protein having 35 phosphorylation sites and a N-glycosylation
site with no transmembrane domain. The protein is expressed in the nucleus,
there is no signal peptide distribution in the whole sequence and the secondary
structure is mainly composed of random coil and α- helix. There were 7 B cell
epitopes, 7 conserved domains and compared with other birds, JAZF1 is 95.61%
similar. In summary, from the analysis we came to conclude that the amino acid
sequence 64-80aa, 91-108aa, 136-151aa and 179-187aa can be selected as antigen
sites and among which 136-151aa may be the best. This study lays a good
foundation for follow-up experiments, which then provides powerful conditions
for pathological detection of pulmonary vascular remodeling and gene drug
therapy of ascites syndrome in broilers.
To Cite This Article:
Guo FP, Liu P, Guo X, Li G, Cheng S, Zou Z, Hou X, Latigo V, Li L, Hu GL and Liu
P, 2020. Bioinformatics analysis of JAZF1 gene in broilers with ascites
syndrome. Pak Vet J.
http://dx.doi.org/10.29261/pakvetj/2020.072