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Association of Tuberculosis with TLR-9 Gene polymorphism and C-Reactive Protein Levels in Blood of Humans and Animals
Muhammad Qasim Ul Hassan Faraz1, Muhammad Tariq Javed1*, Irum Javed2, Sami Ullah Khan Bahadur1, Riaz Hussain3, Narmeen Tariq4, Aziz ur Rehman5, Farkhanda Manzoor6, Shaza Zarnab1, Aisha Khatoon1, Muhammad Hunain Ahmad1 and Aira Tariq7

 1 Department of Pathology, University of Agriculture, Faisalabad Pakistan
Department of Biochemistry, Sardar Bahadur Khan Women’s University, Quetta, Pakistan
University College of Veterinary and Animal Science, The Islamia University of Bahawalpur, Pakistan
Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan
Department of Pathobiology, College of Veterinary and Animal Sciences, Jhang, Pakistan
Department of Zoology, Lahore College for Women University, Lahore Pakistan
Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan
*Corresponding author:


Tuberculosis (TB) is an important bacterial zoonotic disease, causing mortality in humans and animals every year. Pakistan is ranked among top ten in the world based on the prevalence of tuberculosis in humans. Susceptibility to the disease has been linked with genetic variation in TLR-9 gene. Similarly, higher levels of C reactive protein have been reported in TB patients. This study was conducted to investigate the demography of tuberculosis in humans and to explore the association of TLR-9 genes and C-reactive protein levels in patients with tuberculosis in Faisalabad population. The study was also carried out on dairy animals. For TLR-9 gene polymorphism, DNA was extracted, amplified by PCR and fragmented by using restriction enzyme (Bst NI) and visualized after gel electrophoresis. To determine the C-reactive protein concentration, serum samples were sent to the commercial laboratory for ELISA. The demographic analysis showed that majority (P<0.05) of the patients had age between 18-50, height between 5.6-6.0 ft and weight between 50-60 kg. Furthermore, the majority were uneducated, un-employed or belonged to low-income status. Among tuberculosis patients, 86% were smokers, 32% had diabetes, 24% had cardiac disorders, 2% had HIV, 24% had a family history of TB and 20% had hepatitis. Furthermore, there was no family history of TB. The SNP analysis of TLR-9 gene (1237 C/T) showed no association with tuberculosis. However, the serum C-reactive protein levels were significantly (P<0.05) higher in TB positive cases as compared to controls, in both humans and animals.

To Cite This Article: Faraz MQUH, Javed MT, Javed I, Bahadur SUK, Hussain R, Tariq N, Rehman AU, Manzoor F, Zarnab S, Khatoon A, Ahmad MH and Tariq A, 2021. Association of Tuberculosis with TLR-9 gene polymorphism and C-reactive protein levels in blood of humans and animals. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)