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Protective Effects of Cinnamon, Cinnamaldehyde and Kaempferol against Acetaminophen-Induced Acute Liver Injury and Apoptosis in Mouse Model
Zulfia Hussain1,*, Junaid Ali Khan1, M. Imran Arshad2, Faqir Muhammad1 and Rao Zahid Abbas3
1Institute of Physiology and Pharmacology, University of Agriculture, Faisalabad-38040, Pakistan
2Institute of Microbiology, University of Agriculture, Faisalabad-38040, Pakistan
3Department of Parasitology, University of Agriculture, Faisalabad-38040, Pakistan
*Corresponding author:


Liver diseases are among the major health problems in Pakistan. The present study investigated the mechanism of hepatoprotection by cinnamon, cinnamaldehyde and kaempferol in Acetaminophen (APAP)-induced liver injury. Qualitative phytochemical analysis was performed for standardization of cinnamon ethanolic extract. For in-vivo evaluation, Balb/c mice were administered with cinnamon extract (200 mg/kg i.g.), cinnamaldehyde (10 mg/kg) and kaempferol (10 mg/kg) for 14 days followed by administration of APAP (200 mg/kg i.p.). At the end of trial, mice were dissected and blood, liver and spleen samples were collected for biochemical, histopathological and apoptotic genes expression analysis. Statistical analysis was performed for significance of results. The results showed that the hepatic damage due to APAP administration for 8 hours in mice was apparent with increased severity. Cinnamon extract, cinnamaldehyde and kaempferol pre-treatment suggested ameliorative effects on organ injury induced by APAP by decreasing the elevated serum biochemical activity, total proteins and total bilirubin levels. In addition, APAP exerted severe alteration in liver histopathology without affecting spleen histology along with upregulation of Bad, Bax and Caspase-3 and downregulation of Bcl-2. However, cinnamon, cinnamaldehyde and kaempferol pre-treatment ameliorated APAP-induced liver alterations and apoptosis, possibly through their antioxidant activity. In addition, cinnamaldehyde and kaempferol possessed comparable protective potential at 20-times less dose as compared to cinnamon extract alone, suggesting therapeutic potential at lower dose in APAP-induced liver injury and apoptosis.

To Cite This Article: Hussain Z, Khan JA, Arshad MI, Muhammad F and Abbas RZ, 2020. Protective effects of cinnamon, cinnamaldehyde and kaempferol against acetaminophen-induced acute liver injury and apoptosis in mouse model. Pak Vet J.  


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)