PAKISTAN
VETERINARY
JOURNAL
     
 
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Designing of Multiepitope-based Subunit Vaccine (MESV) against prevalent serotype of Foot and Mouth Disease Virus (FMDV) using Immunoinformatics Approach
 
Rimsha Riaz1, Muhammad Sarwar Khan1*, Faiz Ahmad Joyia1 and Muhammad Anjum Zia2
 

1Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad, Pakistan
2Department of Biochemistry, University of Agriculture Faisalabad, Pakistan
*Corresponding author: sarwarkhan_40@hotmail.com

Abstract   

Foot-and-Mouth Disease (FMD) is causing 100% morbidity in adult animals with a high mortality rate in young animals due to cardiomyopathy. The disease-causing virus (FMDV) has been classified into seven serotypes and several topotypes. Amongst serotypes, the serotype O is predominantly present in Pakistan. A vaccine that should be safer and appears as an alternative to conventional vaccines that have failed to control the disease is required. Keeping in view, a multiepitope-based subunit vaccine (MESV) is designed using computational biology-based approaches. The sequence and structural characterization of capsid proteins and 3Cpro of O PanAsia-II sub-lineage are carried out for screening the antigenic proteins. Further, the B- and T-cell epitopes are selected based on toxicity, antigenicity, allergenicity, conservancy, and topology. The Cytotoxic T lymphocyte (CTL) epitopes are confirmed through molecular interactions with selected bovine alleles. The designed MESV is found stable, hydrophilic, antigenic, non-allergen, exposed extracellularly, non-toxic, and soluble. It will stimulate the protective immune response against the viral challenge as it has shown a strong binding affinity with the TLR4. These results suggest that the developed MESV will prove to be a potential candidate vaccine in controlling the disease against serotype O. For expression and accumulation of stable protein the sequence that encodes the multiepitope-based subunit vaccine is codon-optimized following the codon preference of E. coli and is cloned in pET-28a (+).

To Cite This Article: Riaz R, Khan MS, Joyia FA and Zia MA, 2021. Designing of Multiepitope-based Subunit Vaccine (MESV) against prevalent serotype of Foot and Mouth Disease Virus (FMDV) using immunoinformatics approach. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2021.033

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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