PAKISTAN
VETERINARY
JOURNAL
     
 
previous page   Pak Vet J, xxxx, xx(x): xxx-xxx   next page
 
Locally Prepared Bovine Herpesvirus 1 gE Deleted Vaccine Induced Immunogenicity in Rabbits
 
Hammad Ur Rehman1*, Aamir Ghafoor1*, Masood Rabbani1, Amjad Riaz2, Farhat Nazir Awan3 and Sohail Raza1
 

1Institute of Microbiology, University of Veterinary & Animal Sciences, Lahore, Pakistan; 2Department of Theriogenology, University of Veterinary & Animal Sciences, Lahore, Pakistan; 3Provincial Diagnostic Laboratory, Livestock and Dairy Development, Punjab, 16-Cooper Road, Lahore, 54000
*Corresponding author: hammad.rehman@uvas.edu.pk; aamir.ghafoor@uvas.edu.pk

Abstract   

In this study, bovine herpesvirus 1 gE deleted vaccine was prepared from local isolate, by replacing entire gE coding region with enhanced green fluorescent protein (EGFP) for selection. After transfection of MDBK cells with genomic viral DNA and EGFP-bearing gE-deletion plasmid, fluorescent recombinant clones were produced. Deletion of gE gene was confirmed by PCR. BoHV-1ΔgE recombinants formed smaller plaques but replicated in similar titer and kinetics as compared to parental virus (UVAS/2) on MDBK cell lines. In-vivo study showed that locally prepared BoHV-1ΔgE vaccine induced good immune response. For confirmation of seroconversion of gE, serum samples were also tested by gE competition ELISA. The results showed that all rabbits vaccinated with recombinant BoHV-1ΔgE remained negative in gE competition ELISA. The study concluded that locally prepared BoHV-1ΔgE marker vaccine can be candidate vaccine to prevent BoHV-1 infections in Pakistan.

To Cite This Article: Rehman HU, Ghafoor A, Rabbani M, Riaz A, Awan FN and Raza S, 2021. Locally prepared bovine herpesvirus 1 gE deleted vaccine induced immunogenicity in rabbits. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2021.049

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



scopus
 
DOI
 
DOAJ SEAL