PREVALENCE AND IN VITRO ANTIBIOGRAM OF
BACTERIA ASSOCIATED WITH OMPHALITIS IN CHICKS
M. IQBAL,
I.
A. SHAH, A. ALI, M. A. KHAN AND S. JAN
Veterinary Research
Institute, NWFP,
Peshawar, Pakistan
Abstract
The present study was conducted for the isolation of bacterial agents and in
vitro antibiogram of the isolates from the yolk and visceral organs of up to one
week old broiler and layer chicks suffering from omphalitis. Samples from the
yolk and visceral organs were aseptically collected and cultured on various
selective and differential media. The isolated organisms were identified and
subjected to commonly used antibiotics for determination of antibiogram. During
the period of 39 months (May, 2002 to August, 2005), 330 samples from yolk and
visceral organs were taken from those chicks which were not medicated with
antibiotics, as per history provided by the poultry farmers. Among these,
223(68%) showed bacterial growth. Various bacteria isolated were Escherichiacoli (47.93%), proteus (5.87%), mixed infection (3.59%), streptococci
(2.89%), klebsiella (1.79%), salmonella (0.5%), staphylococci (0.5%),
pseudomonas (0.5%), pasteurella (0.5%) and yarseinia (0.5%). The antibiotics
found most effective were Norfloxacin (45.3%), Enrofloxacin (36.81%), Gentamicin
(33.21%), Chloramphenicol (33.21%), Cephradin (25.1%), Augmentine (24.7%),
Kanammycin (17.5%) and Ampicillin (12.1%).