GOAT MILK WHEY AS A FOETAL CALF SERUM SUBSTITUTE FOR
THE GROWTH OF CHICKEN EMBRYO FIBROBLASTS
Munazza Shauket*, M. Ashfaque, Iftikhar Hussain
and T.M.Chaudhary*
*Nuclear Institute for Agriculture and Biology,
P.0.
Box
128,
Jhang Road, Faisalabad; Department of Veterinary Microbiology,
University of Agriculture, Faisalabad, Pakistan
Abstract
Hank’s balanced salt solution (HBSS) was
supplemented with 5, 10, 15, 20 and 25% concentration of goat milk whey for the
growth of chicken embryo fibroblasts. The tissue culture a flask in which media
was supplemented with 25% concentration of goat milk whey supported the growth
of chicken embryo fibroblasts. That growth was comparable to the growth observed
in tissue culture flasks having 5 and 10% of foetal calf serum. No growth was
observed in which foetal calf serum or goat milk whey was not supplemented in
the medium.