Abstract   

A Salmonella isolate of poultry origin was grown at different adjusted p1-i LB-Broth and growth was monitored spectrophotometerically up to 96 hours. Maxinium growth was observed in the medium at p1-I 7.4, while the nonnally used LB-Broth having the pH 6.7 showed diminished growth. No growth was observed at pH 9.2. LB-Broth at different pH values were prepared and after inoculation and incubation at 37 for 24 hours, each of the saniple of different pH was analyzed for the whole cell protein protiles using SDS-PAGE. Comparisons of whole cell protein profiles of different pH samples collected at definite intervals of time were analyzed. The results showed that after 4.5 hours a 24 kd band was absent at pH 5.0. Similarly a 43 kd band was absent at pH 6.7, 5.0 and 8.6. After 6 hours, a 73 kd band was present at pH 8.6 while absent in other pH values. At the same time a 54 kd band was absent at pH 5.5 and 7.4.