Abstract   

The present study was undertaken to develop dot-blot ELISA (DOT-ELISA), a field oriented diagnostic test to detect infectious bursal disease virus (IBDV). The DOT-ELISA, using nitrocellulose membrane, was standardized and field specimens collected from sick birds, apparently infected with IBDV, were analyzed. Efficacy of DOT-ELISA was compared with agar gel precipitation test (AGPT). The DOT-ELISA was found to be more sensitive diagnostic tool than AGPT, indicating the suitability of DOT-ELISA as rapid field test for the detection of IBDV during early stage of infection.