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USE OF DOT-ELISA FOR THE DIAGNOSIS OF INFECTIOUS BURSAL DISEASE IN CHICKENS
Jawaria Sultana, Manzoor Hussain1, Salman A. Malik and Khalid Naeem1 

Department of Biological Sciences, Quad-I-Azam University, Islamabad; 1Animal Sciences Institute, National Agricultural Research Centre, Park Road, Islamabad, Pakistan

Abstract   

The present study was undertaken to develop dot-blot ELISA (DOT-ELISA), a field oriented diagnostic test to detect infectious bursal disease virus (IBDV). The DOT-ELISA, using nitrocellulose membrane, was standardized and field specimens collected from sick birds, apparently infected with IBDV, were analyzed. Efficacy of DOT-ELISA was compared with agar gel precipitation test (AGPT). The DOT-ELISA was found to be more sensitive diagnostic tool than AGPT, indicating the suitability of DOT-ELISA as rapid field test for the detection of IBDV during early stage of infection.

 
 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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