DETECTION OF CRYPTOSPORIDIUM OOCYSTS AND SERUM
IMMUNOGLOBULIN G (lgG) ANTIBODIES IN NATURALLY INFECTED CALVES
Rahmatullah Rind, A.J. Probert1 and
M.I. Rind2
Department of Veterinary Microbiology, Faculty of
Animal Husbandry and Veterinary Sciences, Sindh Agriculture University,
Tandojam; 1University of North Wales, Bangor, UK; 2Department
of Poultry Husbandry and Veterinary Sciences,
SindhAgricultureUniversity,
Tando Jam,
Pakistan
Abstract
Sixty three faecal as well as blood samples from
a group of 15 young Friesian calves under 2 months of age at Aber Farm Bangor,
U.K. were collected on monthly basis and examined for the presence of
Cryptosporidium oocysts and serum immunoglobulin G (IgG) antibodies, Twelve
(19.23 %) were found positive with Cryptosporium species while in 5 (7.9 %)
faecal samples both Cryptosporidium and Eimeria were present but 46 (73.0 %)
samples were negative. In 9 out of 12 (75.0 %) cases where Cryptosporidium
ocysts were present, a positive IF AT was observed while in 4 out of 5 (80.0 %)
positives were seen in the presence of both Cryptosporium and Eimeria oocysts.
In contrast only 6 out of 46 (13.1%) cases, a positive IFAT was also seen when
no oocysts were recorded. Oocysts fluoresced brightly with positive serum
samples and only faintly or not at all with the negative samples or the
conjugate alone.