ADAPTATION OF EIMERIA TENELLA (LOCAL ISOLATE) SPOROZOITES IN CHICKEN EMBRYOS

Masood Akhtar, M. M. Ayaz, C.S. Hayat, M. Ashfaq¹ and I. Hussain¹

Department Veterinary Parasitology, and ¹Department of Veterinary Microbiology, University of Agriculture, Faisalabad

Abstract   

    Emieria (E) tenella ( local isolate} oocysts were recovered from the cases of chicken coccidiosis. Oocysts were sporulated in 2.5 per cent potassium dichromate solution at 37°C with 60 per cent humidity. Excystation of the sporulated oocysts was done with sodium hypochlorite. Sporozoites were released by treating with 1 percent Trypsin ( 1:250) followed by sodium taurocholate at 41 ºC, The final concentrations of the sporozoites were maintained at 1.8x 10³-2.0 x 10³per 0.1 ml. Ten chicken embryos (12 days old) were inoculated each with 0.1 mi suspension of sporozoites into the chorio-allantoic membrane (CAM) along with penicillin and streptomycin, The embryos were maintained at 41ºC with daily candling. Death of the embryo occurred on 5th day post inoculation in six out of ten embryos. Severe hemorrhages were seen on all dead embryos. Total numbers of oocysts harvested from the CAM were 6.1 x 10⁴-7.2 x 10⁴ per ml. The remaining four embryos died on the 7th day post-inoculation and had mild haemorrhages. Total numbers of oocysts harvested were 3.0 x 10⁴-3.5x10⁴