ESTIMATION OF SERUM ALKALINE PHOSPHATASE, CHOLESTEROL, CALCIUM AND PHOSPHORUS DURING PRE-LA YING AND LAYING CONDITIONS IN DIFFERENT STRAINS OF CHICKENS

Bashir Mahmood Bhatti, Tanzeela Talat and Rozina Sardar

Abstract   

    In order to estimate serum alkaline phosphatase, cholesterol, calcium and phosphorus during pre-laying and laying reproductive conditions, 60 hens of Desi, Fayoumi, Cross (Rhode Island Red X Fayoumi} and Nick Chick strains were maintained for one year. Five random blood samples from each strain were collected and analyzed during both pre egg laying and egg laying physiological conditions. It was observed that alkaline phosphatase activity increased significantly (P<0.05) during laying condition. Serum cholesterol remained unaffected by both the strain difference and laying condition. Serum calcium and phosphorus levels increased (P<0.05) during laying condition. The interaction of strain and stage of laying condition was found to exert significant (P<0.05) effect on serum calcium levels. The study showed that the availability of calcium and phosphorus in requisite quantities be provided in diet of laying hens to ensure sustained and quality egg production.