STANDARDIZA TION OF ENZYME LINKED IMMUNOSORBENT ASSAY FOR DETERMINATION OF SEROLOGICAL RESPONSE OF CHICKENS TO HYDROPERICARDIUM SYNDROME VACCINE

Kamal.T.. K. Naeem^l. P. Das and A. Rahim^l

Abstract   

    The present study was conducted to standardize the Enzyme Linked Immunosorbent Assay (EL/SA) for the assessment of serological response of vaccinated chicks with avian adenovirus-4 (AA V-4 ) .The AA V-4 was grown on chicken embryo liver cell culture after plaque purification. The stock virus was utilized to standardize, ELISA and preparation of experimental oil-based hydropericardium syndrome vaccine (OBHPSV) and aqueous base hydropericardium syndrome vaccine (ABHPSV). Optimum ELISA titres (87.16) were recorded for 1:64 dilution of antigen 1:80 dilution of test serum and 1:1000 dilution of conjugate. Serological response of chicks vaccinated at different age groups was monitored using ELISA and it was interred that vaccination at 3^rd day of age with OBHPSV and at 10^th day of age with ABHPSV gave maximum titers.