Abstract   

    Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT) were applied for the diagnosis of brucellosis in 240 buffaloes maintained at organized livestock farms in Punjab, to measure their comparative efficacy. Based on RBPT and SAT, 11.25 (n=27) and 10.42 percent (n=25) buffaloes were found seropositive, 11.67 (n 28) and 4.58 percent (n= 11) animals showed doubtful results, while 77.08 (n= 185) and 85 percept (n= 204) animals were found seronegative, respectively. Rose Bengal Plate Test detected higher percentages of seropositive, doubtful and seronegative cases than those detected by Serum Agglutination Test, which showed lower percentages or seropositive, doubtful and seronegative cases. This study indicated that SAT is more sensitive and reliable diagnostic test for the detection of Brucella aborlus, antibodies in buffaloes

Key words: Buffalos, efficacy, RBPT, SAT, brucellosis