Abstract

    An experimental study was conducted on 12 healthy adult dogs to determine the best method for anastomosis of colon. An appositional 2 layer (APP-2) suture pattern was compared with the inverting 2 layer (INV-2) suture pattern for end-to-end anastomosis. Vicryl^R (size 3-0) was used for anastomosis. At day 10 after surgery, all anastomotic lines were apposed but the mucosa did not cover the lumen of the anastomosis with APP-2 layer suture pattern. The specimens closed w1th INV-2 layer suture pattern showed more pronounced inversion of all layers and the cut ends of the mucosa and submucosa at the tip of told were apposed by a thick plug of fibrous connective tissue. At day 20, the mucosa covered the internal surface and bridged the narrow detect with a single layer of epithelial cells with APP-2 layer suture pattern. The specimens closed With INV-2 layer suture pattern showed slight inversion of the serosa and smooth. muscle caused moderate thickening of the wall. Histopathogical differences between samples collected at 30 days were associated mostly with the maturation process. The current study showed the superiority of the APP-2 layer suture pattern for colonic end-to-end anastomosis in terms of better apposition of the anastomotic layers and better anastomotic healing over the INV -2 layer suture pattern.

Key words: Suturing techniques, end-to-end anastomosis, colon, dogs.