BACTERIAL FLORA OF HATCHERY ENVIRONMENT AND THEIR
IN-VITRO SUSCEPTIBILITY TO ANTIMICROBIAL AGENTS
F. M.
Khan, H. Afzal and F. Deeba
Department of Veterinary Microbiology, University
of
Agriculture,
Faisalabad,
Pakistan
Abstract
Four hatcheries, located in and around Faisalabad, were sampled a day before hatch out in six batches
for environmental bacterial flora. Hatchery air, egg-shell surface, surfaces of
selected locations and water supply samples were taken for this purpose. The
percent (relative) occurrence of various bacterial species recovered from
hatchery environment revealed that Bacillus subtilis was the predominant
isolate (26.93%). followed by Escherichia coli (24.08%),
Staphylococcus epidermidis (16.32%), Staphylococcus aureus (8.16%),
Paratyphoid salmonellae (6.93%), Pseudomonas aeruginosa (4.48%),
Citrobacter jreundii (4.08%), Enterococcus faecalis (3.26%),
Klebsiella pneumoniae (3.26%), Bordetella avium (1.63%) and
Proteus vulgaris (0.81%). In second part of the study, bacterial isolates
were subjected to in-vitro antibiotic sensitivity to 8 antibiotics of common
poultry use. It was found that 98.92, 79.56. 65.59, 61.29, 61.29, 61.29, 53.76
and 38.70 percent of bacterial isolates were sensitive to Norfloxacin,
Gentamicin, Neomycin, Chloramphenicol, Doxycycline, Flumequine, Erythromycin,
and Ampicillin, respectively. In the final part of the study, bacterial isolates
were tested for resistance to 3 commerical hatchery disinfectants (TH4®,
Aldekol Des® 0.2, and Bromosept 10% soln. ®. Only 3.22% of the isolates showed
resistance at manufacturer's recommended dilution (MRD) levels while 11.82% of
the isolates showed resistance at concentrations below the MRD levels.