M. K. Mansoor, S. U. Rahman, I. Hussain, M. H. Rasool and M. A. Zahoor

Department of Veterinary Microbiology, University of Agriculture Faisalabad, Pakistan

    An indirect enzyme-linked immunosorbent assay (ELISA) was performed for the detection of antibodies against Egg Drop Syndrome (EDS) virus.  Virus identification was done through   haemaggluti- nation inhibition (HI) test using known antisera. Antichicken immunoglobulins were raised in goats and purified by ammonium sulphate precipitation technique. These goat-antichicken immunoglobulins were conjugated with horseradish peroxidase. Twenty-seven serum samples were collected from a layers flock vaccinated against EDS and specific antibodies were determined by using a horseradish conjugate.