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PREPARATION OF CONJUGATE FOR USE IN AN ELISA  FOR HUMORAL IMMUNE RESPONSE AGAINST EGG DROP SYNDROME VIRUS IN LAYER CHICKS

M. K. Mansoor, S. U. Rahman, I. Hussain, M. H. Rasool and M. A. Zahoor

Department of Veterinary Microbiology, University of Agriculture Faisalabad, Pakistan

Abstract

    An indirect enzyme-linked immunosorbent assay (ELISA) was performed for the detection of antibodies against Egg Drop Syndrome (EDS) virus.  Virus identification was done through   haemaggluti- nation inhibition (HI) test using known antisera. Antichicken immunoglobulins were raised in goats and purified by ammonium sulphate precipitation technique. These goat-antichicken immunoglobulins were conjugated with horseradish peroxidase. Twenty-seven serum samples were collected from a layers flock vaccinated against EDS and specific antibodies were determined by using a horseradish conjugate.  

Key words: Egg drop syndrome, ELISA, antichicken antibodies, humoral immune response
 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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