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PRODUCTION OF HYPERIMMUNE SERUM AGAINST INFECTIOUS BURSAL DISEASE VIRUS IN RABBITS

I. Hussain, M. H. Rasool and M. S. Mahmood

Department of Microbiology, University of Agriculture, Faisalabad, Pakistan

Abstract

    The early and accurate diagnosis of infectious bursal disease requires known hyperimmune serum against the disease. In this study, an attempt was made to raise anti-infectious bursal disease virus hyperimmune serum in rabbits. The infectious bursal disease (IBD) virus was isolated from field outbreaks, identified, purified, titrated and its infectivity titre was determined in embryonated eggs. Three different semi purified and purified doses were prepared for inoculation in rabbits: (i) chloroform treated IBD virus; (ii) ultra centrifuged and resuspended virus using sucrose as gradients and (iii) pelleted and resuspended virus with addition of incomplete Freund's adjuvant. Killed IBD virus vaccine (Cevac IBDK) was used as 4th inoculum. It was observed that the serum collected after series of inoculation of 1st and 2nd inoculum provided maximum antibody titre upto log26 and Log29, respectively. However, the serum collected after series of injection of 3rd and 4th inoculum gave maximum indirect haemagglutination (IHA) titer (log210). This study suggested that live antigen containing incomplete Freund's adjuvant provided better immune response and may be a good choice for raising a hyperimmune serum against IBD virus in rabbits.

Key words: Hyperimmune serum, infectious bursal disease, incomplete Freund's adjuvant, rabbits

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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