PAKISTAN
VETERINARY
JOURNAL
     
 
previous page   Pak Vet J, 2008, 28(2): 63-67   next page
 

MOLECULAR DIAGNOSIS OF RINDERPEST AND PESTE DES PETITS RUMINANTS VIRUS USING TRIZOL REAGENT

U. FAROOQ, Q. MAHMOOD KHAN1 AND T. BARRETT2

Animal Health Laboratories, National Agricultural Research Centre, Park Road, Islamabad.
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan;
2
Institute of Animal Health, Pirbright, UK

Abstract

    Reverse transcription polymerase chain reaction was standardized to diagnose and differentiate between rinderpest (RP) and peste des petits ruminants (PPR) viruses. The RNA of these viruses was isolated using trizol reagent. The P and N-gene primers amplified DNA of RP and PPR viruses, giving 429bp and 238bp, respectively in regular PCR. The F-gene specific primers amplified RP and PPR viruses giving DNA product of 372bp in regular PCR, while F-gene nested primers differentially amplified RPV and PPRV giving product of 309bp and 235bp, respectively in nested PCR.  

Key words: Rinderpest, PRR, molecular diagnosis
 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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