MOLECULAR DIAGNOSIS OF RINDERPEST AND PESTE DES
PETITS RUMINANTS VIRUS USING TRIZOL REAGENT
U. FAROOQ, Q. MAHMOOD KHAN1 AND T.
BARRETT2
Animal Health Laboratories, National Agricultural
Research Centre, Park Road, Islamabad. National Institute for Biotechnology and
Genetic Engineering (NIBGE), Faisalabad, Pakistan; 2 Institute of
Animal Health, Pirbright, UK
Abstract
Reverse transcription polymerase chain reaction was standardized to
diagnose and differentiate between rinderpest (RP) and peste des petits
ruminants (PPR) viruses. The RNA of these viruses was isolated using
trizol reagent. The P and N-gene
primers amplified DNA of RP and PPR viruses, giving 429bp and 238bp,
respectively in regular PCR. The F-gene specific primers amplified RP
and PPR viruses giving DNA product of 372bp in regular PCR, while F-gene
nested primers differentially amplified RPV and PPRV giving product of
309bp and 235bp, respectively in nested PCR.