OVICIDAL AND LARVICIDAL PROPERTIES OFADHATODA VASICA (L.)EXTRACTS AGAINST GASTROINTESTINAL
NEMATODES OF SHEEP IN VITRO
I. R. M.
AL-SHAIBANI, M. S. PHULAN, A. ARIJOAND T. A. QURESHI 1
Department of
Veterinary Parasitology; 1Department of Veterinary Pharmacology, SindhAgricultureUniversity,
Tando Jam, Pakistan
Abstract
The main objective of this study was to evaluate the anthelmintic
activity of Adhatoda vasica (Acanthaceae) in vitro against
the gastrointestinal nematodes of sheep. The aqueous and ethanolic
extracts of Adhatoda vasica aerial parts were evaluated by egg
hatching and larval development assays. The aqueous and ethanolic
extracts at 25-50 mg/ml concentrations exhibited ovicidal and larvicidal
(p<0.05) activity against gastrointestinal nematodes. The plant extracts
showed dose-dependent inhibition (P<0.05). The ethanolic extract at the
concentration of 50.0 mg/ml was more effective in inhibiting egg
hatching and larval development of gastrointestinal nematodes. The
effective dose (ED50) of aqueous and ethanolic extracts were
determined graphically from linear regression equation with probit
scale, y = 5. The results of this study suggested that Adhatoda
vasica extracts may be useful in the control of gastrointestinal
nematodes of sheep.