EFFECT OF VARIOUS STABILIZERS ON TITRE OF LYOPHILIZED LIVE-ATTENUATED PESTE DES
PETITS RUMINANTS (PPR) VACCINE
M. ASIM, A. RASHID AND A. H. CHAUDHARY
Veterinary Research Institute,
Ghazi Road,
Lahore-Cantt,
Pakistan
Abstract
Lyophilization stabilizes the biological materials by using two overlapping
drying procedure i.e. primary drying by sublimation of the ice crystal from
frozen material and secondary drying or desorption by evaporation of the free
water adsorbed into the dried product. Three different stabilizers i.e.
lactalbumin hydrolysate-sucrose, Weybridge medium and lactalbumin
hydrolysate-manitol were used to lyophilize the Peste des petits ruminants (PPR)
vaccine. Titre of live-attenuated PPR cell culture experimental vaccine was
studied after lyophilization which revealed that PPR vaccine lyophilized with
Weybridge medium was more stable and maintained the virus titre longer than rest
of stabilizers used in the study.
Key words:
Peste des petits ruminants, vaccine, lyophilization.