DIFFERENTIATION OF
CLOSELY RELATED VACCINAL STRAINS OF PASTEURELLA MULTOCIDA USING SODIUM
DODECYL SULPHATE-POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)
IRFAN ULLAH, M.
ABUBAKAR1, R. DURRANI2, R. ANJUM1, N. AYUB AND
Q. ALI1
Department of Microbiology, Faculty of
Biological Sciences, Quaid-i-AzamUniversity,
Islamabad; 1National
Veterinary Laboratory, Park Road,
Islamabad;
2KohatUniversity
of Science and Technology, Kohat,
Pakistan
Abstract
The present study was carried out to observe inter-strain variation at molecular
level in the two vaccinal types (B:3,4 and B:6) of
Pasteurella multocida.
The micro-organisms were identified and characterized by using conventional
microbiological tests. The results of these tests were unable to show any
definite variations between the two vaccinal strains studied. Whole cell
SDS-PAGE profiles showed similarities in the two vaccinal strains. However,
SDS-PAGE profiles of envelope showed differences between the two vaccinal
strains.The 85, 27 kDa major and 70, 40
kDa minor proteins were common in both strains, while the 50, 32 and 29 kDa
major proteins were found in B:3,4 but absent in B:6. SDS-PAGE profiles of outer
membrane protein (OMP) showed that 26 minor bands were common. The 50 and 32 kDa
major proteins were found in B: 3,4 and absent in B:6, while 46 kDa minor
protein was found in B:6 only.
So, the present study concluded that the two vaccinal strains of Pasteurella
multocida had differences in OMP, lipopolysaccharides and whole cell
extracts