CORRELATION BETWEEN HYPO-OSMOTIC SWELLING TEST AND VARIOUS CONVENTIONAL
SEMEN EVALUATION PARAMETERS IN FRESH NILI-RAVI BUFFALO AND SAHIWAL COW BULL
SEMEN
L. A. LODHI, M. ZUBAIR, Z. I. QURESHI,
I.
AHMAD AND H. JAMIL
Department of Theriogenology, University
of AgricultureFaisalabad,
Pakistan
Abstract
The present study was conducted to determine the correlation of hypo-osmotic
swelling test with conventional semen evaluation parameters of fresh semen
collected from two Nili-Ravi buffalo and two Sahiwal cow bulls. A total of 10
pooled samples (each comprising two consecutive ejaculates) from each bull were
collected. Each semen sample was divided into two parts. One part was used for
the evaluation of semen by conventional method, while the other part was
subjected to hypo-osmotic swelling test by using 150 mOsm/L sodium citrate
fructose solution. The mean sperm positive to HOS test was 85.25% both in Nili-Ravi
buffalo and Sahiwal cow bull semen. Statistical analysis of the data revealed a
significant (P<0.05) positive correlation between progressive motility,
morphologically normal spermatozoa, sperm viabilityand percentage of HOS test positive spermatozoa for both species. It was
inferred that HOS test could be a valuable method for routine evaluation of
semen for artificial insemination