PAKISTAN
VETERINARY
JOURNAL
     
 
 
previous page   Pak Vet J, 2009, 29(4): 149-153   next page

 

Immune responses of goats (Shami breed) to vaccination with a full dose, reduced dose and conjunctival dose of Brucevac (Brucella melitensis rev.1) vaccine

F. ALDOMY, M. ALKHAWALDEH1 AND I. B. YOUNIS

Jordan Bioindustries Centre (JOVAC), P.O. Box 43, Amman, 11941; 1Veterinary Department, P. O. Box 2395, Ministry of Agriculture, Amman, Jordan

Abstract

    Three groups of Shami goats were randomly vaccinated with Brucevac (Rev. 1) vaccine. Group 1 was vaccinated subcutaneously with a full dose (1.54 x 109 organisms). Group 2 was vaccinated conjunctively with one eye drop (5.2 x 108 organisms), while Group 3 was injected subcutaneously with a reduced dose (7.1 x 105 organisms) of vaccine. Blood samples were collected before vaccination, two, four, eight, 15 and 24 weeks post vaccination. All samples were tested through CFT, ELISA, SAT and Rose Bengal plate test. All serological tests used detected a higher percentage of vaccinated female kids with a full dose than they did in other groups vaccinated with a reduced dose or with a conjunctival dose of Rev.1 vaccine. The overall results suggested that 100% of animals vaccinated with a conjunctival dose became positive to CFT at two, four, eight, and 15 weeks post vaccination, and then the percentage of seropositive animals declined and became 20% at 24 weeks post inoculation. The conjunctival route of vaccination significantly reduced the intensity and duration of the post vaccination serological response, while makes the use of this vaccine compatible with brucellosis programmes, even when these are based on a test-and–slaughter policy. The overall results showed that Shami goats responded to Rev.1 vaccine in the expected way. The majority of animals were seropositive to the CFT by two weeks after vaccination with higher numbers of seropositive animals in the kids group vaccinated with a full dose of Rev.1 vaccine.

Key words: Brucella melitensis, vaccine, immune response, goats.

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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