COMPARATIVE EFFICACY OF PESTE DES PETITS RUMINANTS (PPR) VACCINES AVAILABLE IN
PAKISTAN
IN SHEEP AND GOATS
M. INTIZAR, M. D.
AHMAD, A. A. ANJUM AND A. HANIF
Department of Microbiology,
University
of Veterinary
and Animal Sciences, Lahore,
Pakistan
Abstract
The efficiency of Peste des petits ruminants virus (PPRV) vaccines available in
Pakistan was evaluated on the basis of the humoral immune response measured by
haemagglutination inhibition (HI) and agar gel immunodiffusion (AGID) testsin sheep (n=60) and goats (n=60). The effect of storage temperature on HA
activity of vaccine virus was measured by holding the vaccine at -20,4,27 and 40°C
for 24 hours. The titer of freshly
prepared vaccine was
1:16
and remained unchanged for 24 hours in the vaccines stored at -20
and 4°C. However, drop in titer (1:2 HA) was
recorded in the vaccine kept at 40°C for 24
hours. The haemagglutination activity of PPR virus constituted in buffer with pH
6.8 and 7.0 was recorded as highest when assay was performed with chicken and
human blood group’O’ erythrocytes (1%). The
lowest titer was recorded when vaccine was reconstituted in buffer at pH 8.0. After
14th day post vaccination, there was a gradual increase in the
antibody titer till 56th day. Geometric mean titer (GMT) of
antibodies against locally manufactured PPRV vaccine was higher (207.9) in
comparison with Pestivec (73.3), a vaccine imported from
Jordan
at 63rd day post vaccination in sheep; the corresponding values in
goats were 147.0 and 48.5, respectively. All animals of control group were
negative for antibodies by both of the diagnostic tests.