PREVALENCE AND ANTIBIOGRAM STUDIES OF SALMONELLA ENTERITIDIS
ISOLATED FROM HUMAN AND POULTRY SOURCES
F. Akhtar,
I.
Hussain*, A. Khan1 and S. U. Rahman
Department of Microbiology; 1Department
Pathology, University of Agriculture, Faisalabad, Pakistan.
*Corresponding author: driftikharuaf@hotmail.com
Abstract
A total of 615 samples including poultry eggs (240), poultry meat (85), poultry
droppings (100), bakery products (65) and stool from human diarrhea cases (125)
were collected from different areas of
Faisalabad,
Pakistan.
The samples enriched in Tetrathionate broth showed turbidity in 206 (33.5%)
samples, and typical Salmonella colonies were found on differential agar media
by using traditional methods in all these cases. Polyvalent antisera were used
to identify Salmonella serovars. Results revealed that overall serovar
entertidis prevalence rate in 206 Salmonella positive samples was 75.24% (155).
Out of 58 isolates of Salmonella recovered from human stool samples, 44 (75.86%)
were S. enteritidis. Isolation
frequency of S. enteritidis from total
isolates (148/206) in poultry sources was 111/148 (75.00%) which indicated the
zoonotic potential of S. enteritidis.
Antimicrobial susceptibility tests were performed by using disk diffusion and
concentration methods. S. enteritidis
isolates showed 100% resistance against bacitracin, erythromycin and novobiocin.
These results indicated possible role of infected poultry and poultry products
as a source of human infection with multiple drug resistant
S. enteritidis strains.
Key words:
Prevalence, Antibiogram,
Salmonellaenteritidis,
Poultry eggs, Human diarrhea