PAKISTAN
VETERINARY
JOURNAL
     
 
previous page   Pak Vet J, 2010, 30(1): 25-28   next page

 

PREVALENCE AND ANTIBIOGRAM STUDIES OF SALMONELLA ENTERITIDIS ISOLATED FROM HUMAN AND POULTRY SOURCES

F. Akhtar, I. Hussain*, A. Khan1 and S. U. Rahman

Department of Microbiology; 1Department Pathology, University of Agriculture, Faisalabad, Pakistan.
*Corresponding author: driftikharuaf@hotmail.com

Abstract

    A total of 615 samples including poultry eggs (240), poultry meat (85), poultry droppings (100), bakery products (65) and stool from human diarrhea cases (125) were collected from different areas of Faisalabad, Pakistan. The samples enriched in Tetrathionate broth showed turbidity in 206 (33.5%) samples, and typical Salmonella colonies were found on differential agar media by using traditional methods in all these cases. Polyvalent antisera were used to identify Salmonella serovars. Results revealed that overall serovar entertidis prevalence rate in 206 Salmonella positive samples was 75.24% (155). Out of 58 isolates of Salmonella recovered from human stool samples, 44 (75.86%) were S. enteritidis. Isolation frequency of S. enteritidis from total isolates (148/206) in poultry sources was 111/148 (75.00%) which indicated the zoonotic potential of S. enteritidis. Antimicrobial susceptibility tests were performed by using disk diffusion and concentration methods. S. enteritidis isolates showed 100% resistance against bacitracin, erythromycin and novobiocin. These results indicated possible role of infected poultry and poultry products as a source of human infection with multiple drug resistant S. enteritidis strains.

Key words: Prevalence, Antibiogram, Salmonella  enteritidis, Poultry eggs, Human diarrhea

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



scopus
 
DOI
 
DOAJ SEAL